Helle S. Waagepetersen
Molecular and Cellular Pharmacology, University of Copenhagen
The research in NeuroMet is focused on energy and amino acid metabolism in the mammalian brain. Primary mouse cell culture systems of neurons and astrocytes from cerebral cortex or cerebellum are extensively employed. In addition, acutely isolated cortical prisms, isolated mitochondria and cell lines are applied as model systems. An array of 3H, 15N and 13C isotopes are utilized in the mapping of metabolic pathways and their regulation. HPLC and mass spectrometry are key analytical tools combined with biochemical assays, protein biochemistry, molecular biology and confocal microscopy.